- Title
- Region-specific changes in mitochondrial D-loop in aged rat CNS
- Creator
- McInerny, Simone C.; Brown, Amanda L.; Smith, Douglas W.
- Relation
- Mechanisms of Ageing and Development Vol. 130, Issue 5, p. 343-349
- Publisher Link
- http://dx.doi.org/10.1016/j.mad.2009.01.008
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2009
- Description
- Impaired mitochondrial oxidative phosphorylation (OXPHOS) is considered a cause of aging. A reduction in mitochondrial DNA (mtDNA) replication and/or transcription may contribute to this OXPHOS diminution. Impairments in the displacement (D) loop, or non-coding, region of the mitochondrial genome, or accumulation of mtDNA mutations, may affect mtDNA replication and transcription. We determined the effects of age on the D-loop and on mtDNA deletion mutations in the spinal cord, medulla, midbrain, cerebellum, striatum, and cerebral cortex of Fischer 344 rats. D-loop, 7S DNA levels were reduced by 3-fold in striatum, 2.5-fold in cortex, and 2-fold in the spinal cord of older animals. We did not detect a population of mtDNA affected by the most prevalent known (ND4-containing) deletions, indicating they do not comprise a significant portion of total mtDNA. However, we detected an age-related and region-specific increase in the common deletion, which comprised 0.0003–0.0007% of total mtDNA. Mitochondrial genome copy number varied between regions, in addition to an overall 18% decrease with age across the whole brain. These results suggest the age-related decline in OXPHOS may be related to a reduction in D-loop function.
- Subject
- mitochondrial genome mutation; hypothesis of aging; real-time quantitative PCR; mitochondrial genome deletions; mitochondrial replication; mitochondrial transcription
- Identifier
- http://hdl.handle.net/1959.13/916912
- Identifier
- uon:8150
- Identifier
- ISSN:0047-6374
- Language
- eng
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